The Energetics and Mass-loss of Mrk33

We present ROSAT HRI X-ray data and optical imaging of the important dwarf starburst Markarian 33. We find an extended, complex, shell-like morphology in the X-ray emission, with an extent of 2.3 x 1.9kpc, coincident with the bright star-forming regions at the centre of the galaxy. The physical extent of this X-ray emission from Mrk 33 is very similar to the observed Halpha emission, and suggests that the bulk of the X-ray emission is coming from an expanding superbubble. We estimate the age and mass of Mrk 33's starburst to be 5.8 Myr and 6.9 x 10^{6} Msolar respectively with the energy injection rate in the central regions of the galaxy being 10^{41} erg/s, while the associated mass-loss rate from the star-forming regions is estimated to be 0.2 Msolar/yr. We suggest that the X-ray emission is predominantly powered by starburst type activity and argue that a blowout in the form of a galactic wind is the most likely fate for Mrk 33 resulting in the loss of most of the galaxy's metal-enriched material and a small fraction (<1 per cent) of the ISM.Comment: 13 pages, 6 figures, accepted for publication in MNRA

Chandra and XMM-Newton Observations of NGC 4214: The Hot Interstellar Medium and the Luminosity Function of Dwarf Starbursts

We present results from Chandra and XMM-Newton X-ray observations of NGC 4214, a nearby dwarf starburst galaxy containing several young regions of very active star-formation. Starburst regions are known to be associated with diffuse X-ray emission, and in this case the X-ray emission from the galaxy shows an interesting morphological structure within the galaxy, clearly associated with the central regions of active star-formation. Of the two main regions of star-formation in this galaxy, X-ray emission associated with the older is identified whereas little is detected from the younger, providing an insight into the evolutionary process of the formation of superbubbles around young stellar clusters. The spectra of the diffuse emission from the galaxy can be fitted with a two temperature component thermal model with kT=0.14keV and 0.52keV, and analysis of this emission suggests that NGC 4214 will suffer a blow-out in the future. The point source population of the galaxy has an X-ray luminosity function with a slope of -0.76. This result, together with those for other dwarf starburst galaxies (NGC 4449 and NGC 5253), was added to a sample of luminosity functions for spiral and starburst galaxies. The slope of the luminosity function of dwarf starbursts is seen to be similar to that of their larger counterparts and clearly flatter than those seen in spirals. Further comparisons between the luminosity functions of starbursts and spiral galaxies are also made.Comment: 16 pages, 12 figures. Accepted for publication in MNRA

The role of the lateral prefrontal cortex and anterior cingulate in stimulus–response association reversals

Many complex tasks require us to flexibly switch between behavioral rules, associations, and strategies. The prefrontal cerebral cortex is thought to be critical to the performance of such behaviors, although the relative contribution of different components of this structure and associated subcortical regions are not fully understood. We used functional magnetic resonance imaging to measure brain activity during a simple task which required repeated reversals of a rule linking a colored cue and a left/right motor response. Each trial comprised three discrete events separated by variable delay periods. A colored cue instructed which response was to be executed, followed by a go signal which told the subject to execute the response and a feedback instruction which indicated whether to ‘‘hold’’ or ‘‘f lip’’ the rule linking the colored cue and response. The design allowed us to determine which brain regions were recruited by the specific demands of preparing a rule contingent motor response, executing such a response, evaluating the significance of the feedback, and reconfiguring stimulus–response (SR) associations. The results indicate that an increase in neural activity occurs within the anterior cingulate gyrus under conditions in which SR associations are labile. In contrast, lateral frontal regions are activated by unlikely/unexpected perceptual events regardless of their significance for behavior. A network of subcortical structures, including the mediodorsal nucleus of the thalamus and striatum were the only regions showing activity that was exclusively correlated with the neurocognitive demands of reversing SR associations. We conclude that lateral frontal regions act to evaluate the behavioral significance of perceptual events, whereas medial frontal–thalamic circuits are involved in monitoring and reconfiguring SR associations when necessary

Chandra & XMM-Newton Observations of NGC5253. Analysis of the X-ray Emission from a Dwarf Starburst Galaxy

We present Chandra and XMM-Newton X-ray data of NGC5253, a local starbursting dwarf elliptical galaxy, in the early stages of a starburst episode. Contributions to the X-ray emission come from discrete point sources and extended diffuse emission, in the form of what appear to be multiple superbubbles, and smaller bubbles probably associated with individual star clusters. Chandra detects 17 sources within the optical extent of NGC5253 down to a completeness level corresponding to a luminosity of 1.5E37 erg/s.The slope of the point source X-ray luminosity function is -0.54, similar to that of other nearby dwarf starburst galaxies. Several different types of source are detected within the galaxy, including X-ray binaries and the emission associated with star-clusters. Comparison of the diffuse X-ray emission with the observed Halpha emission shows similarities in their extent. The best spectral fit to the diffuse emission is obtained with an absorbed, two temperature model giving temperatures for the two gas components of ~0.24keV and ~0.75keV.The derived parameters of the diffuse X-ray emitting gas are as follows: a total mass of \~1.4E6 f^{1/2} Msun, where f is the volume filling factor of the X-ray emitting gas, and a total thermal energy content for the hot X-ray emitting gas of \~3.4E54 f^{1/2} erg. The pressure in the diffuse gas is P/k ~ 1E6f^{-1/2}K/cm3. We find that these values are broadly commensurate with the mass and energy injection from the starburst population. Analysis of the kinematics of the starburst region suggest that the stellar ejecta contained within it can escape the gravitational potential well of the galaxy, and pollute the surrounding IGM.Comment: 19 pages, In Press in MNRA

Control of the stop band of an acoustic double fishnet

Copyright © 2013 Acoustical Society of AmericaThe acoustic transmittance of two closely spaced solid plates, each perforated with a square array of cylindrical holes, exhibits a band of near-perfect acoustic attenuation originating from hybridization between a resonance in the gap separating the plates and pipe resonances in the holes. Displacement of one plate relative to the other, such that the holes are no longer aligned, or an increase in the plate separation leads to an increased center frequency of the stop band. This ability to easily tune the frequency of the stop band may prove advantageous.The authors acknowledge funding of ARJM by EPSRC and Sonardyne International

A simulation environment for studying transcutaneous electrotactile stimulation

Transcutaneous electrical nerve stimulation (TENS) allows the artificial excitation of nerve fibres by applying electric-current pulses through electrodes on the skin's surface. This work involves the development of a simulation environment that can be used for studying transcutaneous electrotactile stimulation and its dependence on electrode layout and excitation patterns. Using an eight-electrode array implementation, it is shown how nerves located at different depths and with different orientations respond to specific injected currents, allowing the replication of already reported experimental findings and the creation of new hypotheses about the tactile sensations associated with certain stimulation patterns. The simulation consists of a finite element model of a human finger used to calculate the distribution of the electric potential in the finger tissues neglecting capacitive effects, and a cable model to calculate the excitation/inhibition of action potentials in each nerve

Dogslife: A web-based longitudinal study of Labrador Retriever health in the UK

<p>Abstract</p> <p>Background</p> <p>Dogslife is the first large-scale internet-based longitudinal study of canine health. The study has been designed to examine how environmental and genetic factors influence the health and development of a birth cohort of UK-based pedigree Labrador Retrievers.</p> <p>Results</p> <p>In the first 12 months of the study 1,407 Kennel Club (KC) registered eligible dogs were recruited, at a mean age of 119 days of age (SD 69 days, range 3 days – 504 days). Recruitment rates varied depending upon the study team’s ability to contact owners. Where owners authorised the provision of contact details 8.4% of dogs were recruited compared to 1.3% where no direct contact was possible. The proportion of dogs recruited was higher for owners who transferred the registration of their puppy from the breeder to themselves with the KC, and for owners who were sent an e-mail or postcard requesting participation in the project. Compliance with monthly updates was highly variable. For the 280 dogs that were aged 400 days or more on the 30^th June 2011, we estimated between 39% and 45% of owners were still actively involved in the project. Initial evaluation suggests that the cohort is representative of the general population of the KC registered Labrador Retrievers eligible to enrol with the project. Clinical signs of illnesses were reported in 44.3% of Labrador Retrievers registered with Dogslife (median age of first illness 138 days), although only 44.1% of these resulted in a veterinary presentation (median age 316 days).</p> <p>Conclusions</p> <p>The web-based platform has enabled the recruitment of a representative population of KC registered Labrador Retrievers, providing the first large-scale longitudinal population-based study of dog health. The use of multiple different methods (e-mail, post and telephone) of contact with dog owners was essential to maximise recruitment and retention of the cohort.</p

X-ray observations of the edge-on star-forming galaxy NGC891 and its supernova SN1986J

We present XMM-Newton observations of NCG891, a nearby edge-on spiral galaxy. We analyse the extent of the diffuse emission emitted from the disk of the galaxy, and find that it has a single temperature profile with best fitting temperature of 0.26 keV, though the fit of a dual-temperature plasma with temperatures of 0.08 and 0.30 keV is also an acceptable fit. There is a considerable amount of diffuse X-ray emission protruding from the disk in the NW direction out to approximately 6 kpc. We analyse the point source population using a Chandra observation, using a maximum likelihood method to find that the slope of the cumulative luminosity function of point sources in the galaxy is -0.77^{+0.13}_{-0.1}. Using a sample of other local galaxies, we compare the X-ray and infrared properties of NGC891 with those of 'normal' and starburst spiral galaxies, and conclude that NGC891 is most likely a starburst galaxy in a quiescent state. We establish that the diffuse X-ray luminosity of spirals scales with the far infra-red luminosity as L_X \propto L_FIR ^{0.87 +- 0.07}, except for extreme starbursts, and NGC891 does not fall in the latter category. We study the supernova SN1986J in both XMM-Newton and Chandra observations, and find that the X-ray luminosity has been declining with time more steeply than expected (L_X \propto t^-3).Comment: 12 pages, 11 figures, accepted for publication in MNRA

Dual predation by bacteriophage and bdellovibrio bacteriovorus can eradicate escherichia coli prey in situations where single predation cannot

Copyright © 2020 Hobley et al. Bacteria are preyed upon by diverse microbial predators, including bacteriophage and predatory bacteria, such as Bdellovibrio bacteriovorus. While bacteriophage are used as antimicrobial therapies in Eastern Europe and are being applied for compassionate use in the United States, predatory bacteria are only just beginning to reveal their potential therapeutic uses. However, predation by either predator type can falter due to different adaptations arising in the prey bacteria. When testing poultry farm wastewater for novel Bdellovibrio isolates on Escherichia coli prey lawns, individual composite plaques were isolated containing both an RTP (rosette-tailed-phage)-like-phage and a B. bacteriovorus strain and showing central prey lysis and halos of extra lysis. Combining the purified phage with a lab strain of B. bacteriovorus HD100 recapitulated haloed plaques and increased killing of the E. coli prey in liquid culture, showing an effective side-by-side action of these predators compared to their actions alone. Using approximate Bayesian computation to select the best fitting from a variety of different mathematical models demonstrated that the experimental data could be explained only by assuming the existence of three prey phenotypes: (i) sensitive to both predators, (ii) genetically resistant to phage only, and (iii) plastic resistant to B. bacteriovorus only. Although each predator reduces prey availability for the other, high phage numbers did not abolish B. bacteriovorus predation, so both predators are competent to coexist and are causing different selective pressures on the bacterial surface while, in tandem, controlling prey bacterial numbers efficiently. This suggests that combinatorial predator therapy could overcome problems of phage resistance. Importance: With increasing levels of antibiotic resistance, the development of alternative antibacterial therapies is urgently needed. Two potential alternatives are bacteriophage and predatory bacteria. Bacteriophage therapy has been used, but prey/host specificity and the rapid acquisition of bacterial resistance to bacteriophage are practical considerations. Predatory bacteria are of interest due to their broad Gram-negative bacterial prey range and the lack of simple resistance mechanisms. Here, a bacteriophage and a strain of Bdellovibrio bacteriovorus, preyed side by side on a population of E. coli, causing a significantly greater decrease in prey numbers than either alone. Such combinatorial predator therapy may have greater potential than individual predators since prey surface changes selected for by each predator do not protect prey against the other predator

In vivo imaging reveals increased eosinophil uptake in the lungs of obese asthmatic patients.

To The Editor: Eosinophils play an important pathogenic role in pulmonary and systemic conditions including eosinophilic asthma and eosinophilic granulomatosis with polyangiitis.1,2 While progress has been made in understanding the mechanisms responsible for the activation of these cells, existing biomarkers of eosinophilic inflammation are indirect and/or invasive and do not always correlate with tissue eosinophilia. Hence, there is a need to develop non-invasive biomarkers of tissue eosinophilia. We have previously demonstrated the capacity of SPECT (single photon emission computed tomography) to quantify neutrophil uptake into the lungs of COPD patients.3 We sought to determine whether this methodology could be used to quantify eosinophil kinetics and pulmonary uptake, which may differ amongst diseases characterized by eosinophilic inflammation. In particular, the role of the eosinophil in asthma with obesity, a distinct asthma endotype associated with increased severity,4 is controversial. We hypothesized that injection of radiolabeled eosinophils, coupled with SPECT/CT, would reveal changes in eosinophil kinetics in patients compared to healthy volunteers.This work was supported by Asthma UK [08/11], the Medical Research Council [grant number MR/J00345X/1], the Wellcome Trust [grant number 098351/Z/12/Z], Cambridge NIHR Biomedical Research Centre, Wellcome Trust Senior Fellowship (to CEB) [grant number WT082265], AirPROM 7th EU Framework grant and Leicester NIHR Biomedical Research Centre